Publications
Featured
Kasap, C., Elemento, O., Kapoor, T.M. (2014), DrugTargetSeqR: a genomics- and CRISPR/Cas9-based method to analyze drug targets. Nature Chemical Biology, 10(8):626-8.
Wacker, S.A., Houghtaling, B.R., Elemento, O., Kapoor, T.M. (2012) Using transcriptome sequencing to identify mechanisms of drug action and resistance. Nature Chemical Biology, 8(3):235-237.
Wacker, S.A., Houghtaling, B.R., Elemento, O., Kapoor, T.M. (2012) Using transcriptome sequencing to identify mechanisms of drug action and resistance. Nature Chemical Biology, 8(3):235-237.
- Determining a drug’s physiological target is a major challenge in chemical biology and drug discovery. Current strategies fall into two broad categories: model organism-based approaches and affinity-based methods (e.g. introducing a chemical handle for pull-downs followed by mass spectrometry). However, many drugs are not active in model organisms and affinity-based approaches are usually only effective when the drug is potent and the target is abundant in vivo...Read More
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Subramanian, R., Ti, S.C., Tan, L., Darst, S.A., Kapoor, T.M. (2013), Marking and measuring single microtubules by PRC1 and kinesin-4. Cell, 145, 377-90. (Highlighted in Developmental Cell, 26, 118)
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Firestone, A.J., Weinger, J.S., Maldonado, M., Barlan, K., Langston, L.D., O'Donnell, M., Gelfand, V.I., Kapoor. T.M.*, Chen, J.K.* (2012) Small-molecule inhibitors of the AAA+ ATPase motor cytoplasmic dynein. Nature, 484, 125-9 [*corresponding authors] (Highlighted in Nature Reviews Drug Discovery, 11, 354; Nature Chemical Biology, 8, 503)
- Examining dynein functions in these diverse dynamic processes has been difficult using conventional approaches. In this report we describe the discovery of ciliobrevins, dihydroquinazolinone-based small molecules that are the first selective inhibitors of cytoplasmic dynein. We show that ciliobrevin treatment disrupts protein trafficking within the primary cilium and leads to shorter and malformed cilia. Consistent with these effects, Hedgehog signaling is blocked by ciliobrevins. These compounds also inhibit proper mitotic spindle assembly and block organelle transport in cultured cells...Read More
Shimamoto, Y., Maeda, Y.T., Ishiwata, S., Libchaber, A.J., Kapoor, T.M. (2011) Insights into the micromechanical properties of the metaphase spindle. Cell, 145, 1062-74. (Highlighted in Cell PaperFlick; Current Biology, 21(18), R688-R690)
- The metaphase spindle must generate nanonewton-scale forces to pull chromosomes apart and must also be robust to withstand opposing forces of equal magnitude. Forces also act on the metaphase spindle to control its position and size. In addition, chromosomes, which are proportionately large cargoes, must be allowed to move through the spindle’s dense cytoskeletal polymer network. The forces associated with these chromosome motions exert deformations within the metaphase spindle. How this essential cellular structure maintains functional fidelity and structural stability in the face of these forces that can vary in magnitude, orientation and time-scales is poorly understood. To address this question we measured the orientation- and timescale-dependent micromechanical properties of the metaphase spindle...Read More
Subramanian, R., Wilson-Kubalek, E.M., Arthur, C.P., Bick, M.J., Campbell, E.A., Darst, S.A., Milligan, R.A., Kapoor, T.M. (2010) Insights into Antiparallel Microtubule Crosslinking by PRC1, a Conserved Nonmotor Microtubule Binding Protein. Cell. 142, 433-43. (Highlighted in Cell, Leading Edge Minireview, 142, 364-7; Nature Reviews Molecular Cell Biology, Research Highlight, 11, 602-603)
- This study represents an important step in our efforts to reconstitute a ‘minimal mitotic spindle’ with purified proteins. The biophysical and structural characterization of the key non-motor microtubule-associated proteins (MAPs) required for cell division lags far behind that of motor proteins (e.g. kinesins). To fill this knowledge gap and to characterize the proteins needed for our reconstitution assays, we focused on PRC1. This non-motor MAP, or its homologs, is needed for normal cell growth in plants and fungi. In metazoans, PRC1 is needed for successful cell division. We combined X-ray crystallography, electron microscopy, and TIRF (total internal reflection fluorescence) microscopy approaches to analyze PRC1’s functions...Read More
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Fuller, B.G., Lampson, M.A., Foley, E.A., Rosasco-Nitcher, S., Le, K.V., Tobelman, P., Brautigan, D.L., Stukenberg, P.T. and Kapoor, T.M. (2008) Midzone Activation of Aurora B in Anaphase Produces an Intracellular Phosphorylation Gradient. Nature, 453, 1132-6.
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Bibliography
2010-2015
Hsia KC, Wilson-Kubalek EM, Dottore A, Hao Q, Tsai KL, Forth S, Shimamoto Y, Milligan RA, Kapoor TM Reconstitution of the augmin complex provides insights into its architecture and function NATURE CELL BIOLOGY 2014 SEP; 16(9):852-863 Full Text | PMID/PMCID
Kasap C, Elemento O, Kapoor TM DrugTargetSeqR: a genomics- and CRISPR-Cas9-based method to analyze drug targets NATURE CHEMICAL BIOLOGY 2014 AUG; 10(8):626-U157 Full Text | PMID/PMCID
He M, Subramanian R, Bangs F, Omelchenko T, Liem KF, Kapoor TM, Anderson KV The kinesin-4 protein Kif7 regulates mammalian Hedgehog signalling by organizing the cilium tip compartment NATURE CELL BIOLOGY 2014 JUL; 16(7):663-+ Full Text | PMID/PMCID
Sikirzhytski V, Magidson V, Steinman JB, He J, Le Berre M, Tikhonenko I, Ault JG, McEwen BF, Chen JK, Sui HX, Pie M,Kapoor TM, Khodjakov A Direct kinetochore-spindle pole connections are not required for chromosome segregation JOURNAL OF CELL BIOLOGY 2014 JUL 21; 206(2):231-243 Full Text | PMID/PMCID
Forth S, Hsia KC, Shimamoto Y, Kapoor TM Asymmetric Friction of Nonmotor MAPs Can Lead to Their Directional Motion in Active Microtubule Networks CELL 2014 APR 10; 157(2): Full Text | PMID/PMCID
Kashyap S, Sandler J, Peters U, Martinez EJ, Kapoor TM Using 'biased-privileged' scaffolds to identify lysine methyltransferase inhibitors BIOORGANIC & MEDICINAL CHEMISTRY 2014 APR 1; 22(7):2253-2260 Full Text |PMID/PMCID
Aoi Y, Sato M, Sutani T, Shirahige K, Kapoor TM, Kawashima SA Dissecting the first and the second meiotic divisions using a marker-less drug-hypersensitive fission yeast CELL CYCLE 2014 APR 15; 13(8):1327-1334 Full Text |PMID/PMCID
Takagi J, Itabashi T, Suzuki K, Shimamoto Y, Kapoor TM, Ishiwata S Micromechanics of the Vertebrate Meiotic Spindle Examined by Stretching along the Pole-to-Pole Axis BIOPHYSICAL JOURNAL 2014 FEB 4; 106(3):735-740 Full Text | PMID/PMCID
Subramanian R, Kapoor TM Slipping past the spindle assembly checkpoint NATURE CELL BIOLOGY 2013 NOV; 15(11):1261-1263 Full Text | PMID/PMCID
Takagi J, Itabashi T, Suzuki K, Kapoor TM, Shimamoto Y, Ishiwata S Using Micromanipulation to Analyze Control of Vertebrate Meiotic Spindle Size CELL REPORTS 2013 OCT; 5(1):44-50 Full Text | PMID/PMCID
Yi JS, Wu XF, Chung AH, Chen JK, Kapoor TM, Hammer JA Centrosome repositioning in T cells is biphasic and driven by microtubule end-on capture-shrinkage JOURNAL OF CELL BIOLOGY 2013 SEP 2; 202(5):779-792 Full Text |PMID/PMCID
Kleiner RE, Ti SC, Kapoor TM Site-Specific Chemistry on the Microtubule Polymer JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2013 AUG 28; 135(34):12520-12523 Full Text | PMID/PMCID
Kleiner RE, Kapoor TM Bumping Up Kinase Activity with an ATP-Derived Neo-Substrate CELL 2013 AUG 15; 154(4):716-718 Full Text | PMID/PMCID
Liu X, Kapoor TM, Chen JK, Huse M Diacylglycerol promotes centrosome polarization in T cells via reciprocal localization of dynein and myosin II PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2013 JUL 16; 110(29):11976-11981 Full Text | PMID/PMCID
Li X, Foley EA, Kawashima SA, Molloy KR, Li YY, Chait BT, Kapoor TM Examining post-translational modification-mediated protein-protein interactions using a chemical proteomics approach PROTEIN SCIENCE 2013 MAR; 22(3):287-295 Full Text | PMID/PMCID
Kawashima SA, Takemoto A, Nurse P, Kapoor TM A Chemical Biology Strategy to Analyze Rheostat-like Protein Kinase-Dependent Regulation CHEMISTRY & BIOLOGY 2013 FEB 21; 20(2):262-271 Full Text | PMID/PMCID
Foley EA, Kapoor TM Microtubule attachment and spindle assembly checkpoint signalling at the kinetochore NATURE REVIEWS MOLECULAR CELL BIOLOGY 2013 JAN; 14(1):25-37 Full Text | PMID/PMCID
Subramanian R, Kapoor TM Building Complexity: Insights into Self-Organized Assembly of Microtubule-Based Architectures DEVELOPMENTAL CELL 2012 NOV 13; 23(5):874-885 Full Text | PMID/PMCID
Kawashima SA, Takemoto A, Nurse P, Kapoor TM Analyzing Fission Yeast Multidrug Resistance Mechanisms to Develop a Genetically Tractable Model System for Chemical Biology CHEMISTRY & BIOLOGY 2012 JUL 27; 19(7):893-901 Full Text | PMID/PMCID
Firestone AJ, Weinger JS, Maldonado M, Barlan K, Langston LD, O'Donnell M, Gelfand VI, Kapoor TM, Chen JK Small-molecule inhibitors of the AAA plus ATPase motor cytoplasmic dynein NATURE 2012 APR 5; 484(7392):125-129 Full Text | PMID/PMCID
Shimamoto Y, Kapoor TM Microneedle-based analysis of the micromechanics of the metaphase spindle assembled in Xenopus laevis egg extracts NATURE PROTOCOLS 2012 APR; 7(5):959-969 Full Text | PMID/PMCID
Wacker SA, Houghtaling BR, Elemento O, Kapoor TM Using transcriptome sequencing to identify mechanisms of drug action and resistance NATURE CHEMICAL BIOLOGY 2012 MAR; 8(3):235-237 Full Text | PMID/PMCID
Li X, Foley EA, Molloy KR, Li YY, Chait BT, Kapoor TM Quantitative Chemical Proteomics Approach To Identify Post-translational Modification-Mediated Protein-Protein Interactions JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2012 FEB 1; 134(4):1982-1985 Full Text | PMID/PMCID
Tan L, Kapoor TM Examining the dynamics of chromosomal passenger complex (CPC)-dependent phosphorylation during cell division PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2011 OCT 4; 108(40):16675-16680 Full Text | PMID/PMCID
Foley EA, Maldonado M, Kapoor TM Formation of stable attachments between kinetochores and microtubules depends on the B56-PP2A phosphatase NATURE CELL BIOLOGY 2011 OCT; 13(10):1265-U216 Full Text | PMID/PMCID
Li X, Kapoor TM An Optical Switch for a Motor Protein CHEMBIOCHEM 2011 OCT 17; 12(15):2265-2266 Full Text |PMID/PMCID
Wacker SA, Kashyap S, Li X, Kapoor TM Examining the Mechanism of Action of a Kinesin Inhibitor Using Stable Isotope Labeled Inhibitors for Cross-Linking (SILIC) JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2011 AUG 17; 133(32):12386-12389 Full Text | PMID/PMCID
Maldonado M, Kapoor TM Moving Right Along: How PP1 Helps Clear the Checkpoint DEVELOPMENTAL CELL 2011 JUN 14; 20(6):733-734 Full Text | PMID/PMCID
Shimamoto Y, Maeda YT, Ishiwata S, Libchaber AJ, Kapoor TM Insights into the Micromechanical Properties of the Metaphase Spindle CELL 2011 JUN 24; 145(7):1062-1074 Full Text | PMID/PMCID
Maldonado M, Kapoor TM Constitutive Mad1 targeting to kinetochores uncouples checkpoint signalling from chromosome biorientation NATURE CELL BIOLOGY 2011 APR; 13(4):475-U291 Full Text | PMID/PMCID
Weinger JS, Qiu MH, Yang G, Kapoor TM A Nonmotor Microtubule Binding Site in Kinesin-5 Is Required for Filament Crosslinking and Sliding CURRENT BIOLOGY 2011 JAN 25; 21(2):154-160 Full Text | PMID/PMCID
Shimamoto Y, Kapoor TM MECHANOREGULATION Cellular seat belts NATURE 2010 NOV 25; 468(7323):518-519Full Text | PMID/PMCID
Subramanian R, Wilson-Kubalek EM, Arthur CP, Bick MJ, Campbell EA, Darst SA, Milligan RA, Kapoor TM Insights into Antiparallel Microtubule Crosslinking by PRC1, a Conserved Nonmotor Microtubule Binding Protein CELL 2010 AUG 6; 142(3):433-443 Full Text | PMID/PMCID
Tseng BS, Tan L, Kapoor TM, Funabiki H Dual Detection of Chromosomes and Microtubules by the Chromosomal Passenger Complex Drives Spindle Assembly DEVELOPMENTAL CELL 2010 JUN 15; 18(6):903-912 Full Text |PMID/PMCID
Wacker SA, Kapoor TM Targeting a kinetochore-associated motor protein to kill cancer cells PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2010 MAR 30; 107(13):5699-5700 Full Text | PMID/PMCID
Li X, Kapoor TM Approach to Profile Proteins That Recognize Post-Translationally Modified Histone "Tails" JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2010 MAR 3; 132(8):2504-+ Full Text | PMID/PMCID
Islam K, Chin HF, Olivares AO, Saunders LP, De La Cruz EM, Kapoor TM A Myosin V Inhibitor Based on Privileged Chemical Scaffolds ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2010; 49(45):8484-8488 Full Text | PMID/PMCID
2004-2009
Houghtaling BR, Yang G, Matov A, Danuser G, Kapoor TM Op18 reveals the contribution of nonkinetochore microtubules to the dynamic organization of the vertebrate meiotic spindle PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2009 SEP 8; 106(36):15338-15343 Full Text | PMID/PMCID
Foley EA, Kapoor TM Chromosome congression: on the bi-orient express NATURE CELL BIOLOGY 2009 JUL; 11(7):787-789 Full Text | PMID/PMCID
Subramanian R, Kapoor T Meiotic Spindle Self-Organization: One Plus One Equals Only One CURRENT BIOLOGY 2009 APR 14; 19(7):R290-R292 Full Text | PMID/PMCID
Itabashi T, Takagi J, Shimamoto Y, Onoe H, Kuwana K, Shimoyama I, Gaetz J, Kapoor TM, Ishiwata S Probing the mechanical architecture of the vertebrate meiotic spindle NATURE METHODS 2009 FEB; 6(2):167-172 Full Text |PMID/PMCID
Kapitein LC, Kwok BH, Weinger JS, Schmidt CF, Kapoor TM, Peterman EJG Microtubule cross-linking triggers the directional motility of kinesin-5 JOURNAL OF CELL BIOLOGY 2008 AUG 11; 182(3):421-428 Full Text | PMID/PMCID
Fuller BG, Lampson MA, Foley EA, Rosasco-Nitcher S, Le KV, Tobelmann P, Brautigan DL, Stukenberg PT, Kapoor TM Midzone activation of aurora B in anaphase produces an intracellular phosphorylation gradient NATURE 2008 JUN 19; 453(7198):1132-U14 Full Text | PMID/PMCID
Yang G, Houghtaling BR, Gaetz J, Liu JZ, Danuser G, Kapoor TM Architectural dynamics of the meiotic spindle revealed by single-fluorophore imaging NATURE CELL BIOLOGY 2007 NOV; 9(11):1233-U45 Full Text
Loncarek J, Kisurina-Evgenieva O, Vinogradova T, Hergert P, La Terra S, Kapoor TM, Khodjakov A The centromere geometry essential for keeping mitosis error free is controlled by spindle forces NATURE 2007 NOV 29; 450(7170):745-U14 Full Text
Brennan IM, Peters U, Kapoor TM, Straight AF Polo-Like Kinase Controls Vertebrate Spindle Elongation and Cytokinesis PLOS ONE 2007 MAY 2; 2(5):? Article e409 Full Text
Kwok BH, Kapoor TM Microtubule flux: drivers wanted CURRENT OPINION IN CELL BIOLOGY 2007 FEB; 19(1):36-42 Full Text
Peters U, Cherian J, Kim JH, Kwok BH, Kapoor TM Probing cell-division phenotype space and Polo-like kinase function using small molecules NATURE CHEMICAL BIOLOGY 2006 NOV; 2(11):618-626 Full Text
Lampson MA, Kapoor TM Targeting protein stability with a small molecule CELL 2006 SEP 8; 126(5):827-829 Full Text
Kwok BH, Kapitein LC, Kim JH, Peterman EJG, Schmidt CF, Kapoor TM Allosteric inhibition of kinesin-5 modulates its processive directional motility NATURE CHEMICAL BIOLOGY 2006 SEP; 2(9):480-485 Full Text
Gaetz J, Gueroui Z, Libchaber A, Kapoor TM Examining how the spatial organization of chromatin signals influences metaphase spindle assembly NATURE CELL BIOLOGY 2006 SEP; 8(9):924-U41 Full Text
Kapoor TM, Lampson MA, Hergert P, Cameron L, Cimini D, Salmon ED, McEwen BF, Khodjakov A Chromosomes can congress to the metaphase plate before biorientation SCIENCE 2006 JAN 20; 311(5759):388-391 Full Text
Lampson MA, Kapoor TM Unraveling cell division mechanisms with small-molecule inhibitors NATURE CHEMICAL BIOLOGY 2006 JAN; 2(1):19-27 Full Text
Khodjakov A, Kapoor T Microtubule flux: What is it good for? CURRENT BIOLOGY 2005 DEC 6; 15(23):R966-R968 Full Text
Mitchison TJ, Maddox P, Gaetz J, Groen A, Shirasu M, Desai A, Salmon ED, Kapoor TM Roles of polymerization dynamics, opposed motors, and a tensile element in governing the length of Xenopus extract meiotic spindles MOLECULAR BIOLOGY OF THE CELL 2005 JUN; 16(6):3064-3076 Full Text
Kapitein LC, Peterman EJG, Kwok BH, Kim JH, Kapoor TM, Schmidt CF The bipolar mitotic kinesin Eg5 moves on both microtubules that it crosslinks NATURE 2005 MAY 5; 435(7038):114-118 Full Text
Cochran JC, Gatial JE, Kapoor TM, Gilbert SP Monastrol inhibition of the mitotic kinesin Eg5 JOURNAL OF BIOLOGICAL CHEMISTRY 2005 APR 1; 280(13):12658-12667 Full Text
Marcus AI, Peters U, Thomas SL, Garrett S, Zelnak A, Kapoor TM, Giannakakou P Mitotic kinesin inhibitors induce mitotic arrest and cell death in taxol-resistant and -sensitive cancer cells JOURNAL OF BIOLOGICAL CHEMISTRY 2005 MAR 25; 280(12):11569-11577 Full Text
Lampson MA, Kapoor TM The human mitotic checkpoint protein BubR1 regulates chromosome-spindle attachments NATURE CELL BIOLOGY 2005 JAN; 7(1):93-+ Full Text
Mitchison TJ, Maddox P, Groen A, Cameron L, Perlman Z, Ohi R, Desai A, Salmon ED, Kapoor TM Bipolarization and poleward flux correlate during Xenopus extract spindle assembly MOLECULAR BIOLOGY OF THE CELL 2004 DEC; 15(12):5603-5615 Full Text
Solecki DJ, Model L, Gaetz J, Kapoor TM, Hatten ME Par6 alpha signaling controls glial-guided neuronal migration NATURE NEUROSCIENCE 2004 NOV; 7(11):1195-1203 Full Text
Kwok BH, Yang JG, Kapoor TM The rate of bipolar spindle assembly depends on the microtubule-gliding velocity of the mitotic kinesin Eg5 CURRENT BIOLOGY 2004 OCT 5; 14(19):1783-1788 Full Text
Cochran JC, Sontag CA, Maliga Z, Kapoor TM, Correia JJ, Gilbert SP Mechanistic analysis of the mitotic kinesin Eg5 JOURNAL OF BIOLOGICAL CHEMISTRY 2004 SEP 10; 279(37):38861-38870 Full Text
Gaetz J, Kapoor TM Dynein/dynactin regulate metaphase spindle length by targeting depolymerizing activities to spindle poles JOURNAL OF CELL BIOLOGY 2004 AUG 16; 166(4):465-471 Full Text
Lampson MA, Renduchitala K, Khodjakov A, Kapoor TM Correcting improper chromosome-spindle attachments during cell division NATURE CELL BIOLOGY 2004 MAR; 6(3):232-237 Full Text
Peters U, Kapoor TM New probes for microtubule dynamics CHEMISTRY & BIOLOGY 2004 JAN; 11(1):14-16 Full Text
2001-2003
Garrett S, Kapoor TM Microtubule assembly: Catastrophe factors to the rescue CURRENT BIOLOGY 2003 OCT 14; 13(20):R810-R812 Full Text
Canman JC, Cameron LA, Maddox PS, Straight A, Tirnauer JS, Mitchison TJ, Fang GW, Kapoor TM, Salmon ED Determining the position of the cell division plane NATURE 2003 AUG 28; 424(6952):1074-1078 Full Text
Khodjakov A, Copenagle L, Gordon MB, Compton DA, Kapoor TM Minus-end capture of preformed kinetochore fibers contributes to spindle morphogenesis JOURNAL OF CELL BIOLOGY 2003 MAR 3; 160(5):671-683 Full Text
Hotha S, Yarrow JC, Yang JG, Garrett S, Renduchintala KV, Mayer TU, Kapoor TM HR22C16: A potent small-molecule probe for the dynamics of cell division ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2003; 42(21):2379-2382 Full Text
Garrett S, Auer K, Compton DA, Kapoor TM hTPX2 is required for normal spindle morphology and centrosome integrity during vertebrate cell division CURRENT BIOLOGY 2002 DEC 10; 12(23):2055-2059 Full Text
Maliga Z, Kapoor TM, Mitchison TJ Evidence that monastrol is an allosteric inhibitor of the mitotic kinesin Eg5 CHEMISTRY & BIOLOGY 2002 SEP; 9(9):989-996 Full Text
Kapoor TM, Compton DA Searching for the middle ground: mechanisms of chromosome alignment during mitosis JOURNAL OF CELL BIOLOGY 2002 MAY 13; 157(4):551-556 Full Text
Kapoor TM, Mitchison TJ Eg5 is static in bipolar spindles relative to tubulin: evidence for a static spindle matrix JOURNAL OF CELL BIOLOGY 2001 SEP 17; 154(6):1125-1133 Full Text
Hsia KC, Wilson-Kubalek EM, Dottore A, Hao Q, Tsai KL, Forth S, Shimamoto Y, Milligan RA, Kapoor TM Reconstitution of the augmin complex provides insights into its architecture and function NATURE CELL BIOLOGY 2014 SEP; 16(9):852-863 Full Text | PMID/PMCID
Kasap C, Elemento O, Kapoor TM DrugTargetSeqR: a genomics- and CRISPR-Cas9-based method to analyze drug targets NATURE CHEMICAL BIOLOGY 2014 AUG; 10(8):626-U157 Full Text | PMID/PMCID
He M, Subramanian R, Bangs F, Omelchenko T, Liem KF, Kapoor TM, Anderson KV The kinesin-4 protein Kif7 regulates mammalian Hedgehog signalling by organizing the cilium tip compartment NATURE CELL BIOLOGY 2014 JUL; 16(7):663-+ Full Text | PMID/PMCID
Sikirzhytski V, Magidson V, Steinman JB, He J, Le Berre M, Tikhonenko I, Ault JG, McEwen BF, Chen JK, Sui HX, Pie M,Kapoor TM, Khodjakov A Direct kinetochore-spindle pole connections are not required for chromosome segregation JOURNAL OF CELL BIOLOGY 2014 JUL 21; 206(2):231-243 Full Text | PMID/PMCID
Forth S, Hsia KC, Shimamoto Y, Kapoor TM Asymmetric Friction of Nonmotor MAPs Can Lead to Their Directional Motion in Active Microtubule Networks CELL 2014 APR 10; 157(2): Full Text | PMID/PMCID
Kashyap S, Sandler J, Peters U, Martinez EJ, Kapoor TM Using 'biased-privileged' scaffolds to identify lysine methyltransferase inhibitors BIOORGANIC & MEDICINAL CHEMISTRY 2014 APR 1; 22(7):2253-2260 Full Text |PMID/PMCID
Aoi Y, Sato M, Sutani T, Shirahige K, Kapoor TM, Kawashima SA Dissecting the first and the second meiotic divisions using a marker-less drug-hypersensitive fission yeast CELL CYCLE 2014 APR 15; 13(8):1327-1334 Full Text |PMID/PMCID
Takagi J, Itabashi T, Suzuki K, Shimamoto Y, Kapoor TM, Ishiwata S Micromechanics of the Vertebrate Meiotic Spindle Examined by Stretching along the Pole-to-Pole Axis BIOPHYSICAL JOURNAL 2014 FEB 4; 106(3):735-740 Full Text | PMID/PMCID
Subramanian R, Kapoor TM Slipping past the spindle assembly checkpoint NATURE CELL BIOLOGY 2013 NOV; 15(11):1261-1263 Full Text | PMID/PMCID
Takagi J, Itabashi T, Suzuki K, Kapoor TM, Shimamoto Y, Ishiwata S Using Micromanipulation to Analyze Control of Vertebrate Meiotic Spindle Size CELL REPORTS 2013 OCT; 5(1):44-50 Full Text | PMID/PMCID
Yi JS, Wu XF, Chung AH, Chen JK, Kapoor TM, Hammer JA Centrosome repositioning in T cells is biphasic and driven by microtubule end-on capture-shrinkage JOURNAL OF CELL BIOLOGY 2013 SEP 2; 202(5):779-792 Full Text |PMID/PMCID
Kleiner RE, Ti SC, Kapoor TM Site-Specific Chemistry on the Microtubule Polymer JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2013 AUG 28; 135(34):12520-12523 Full Text | PMID/PMCID
Kleiner RE, Kapoor TM Bumping Up Kinase Activity with an ATP-Derived Neo-Substrate CELL 2013 AUG 15; 154(4):716-718 Full Text | PMID/PMCID
Liu X, Kapoor TM, Chen JK, Huse M Diacylglycerol promotes centrosome polarization in T cells via reciprocal localization of dynein and myosin II PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2013 JUL 16; 110(29):11976-11981 Full Text | PMID/PMCID
Li X, Foley EA, Kawashima SA, Molloy KR, Li YY, Chait BT, Kapoor TM Examining post-translational modification-mediated protein-protein interactions using a chemical proteomics approach PROTEIN SCIENCE 2013 MAR; 22(3):287-295 Full Text | PMID/PMCID
Kawashima SA, Takemoto A, Nurse P, Kapoor TM A Chemical Biology Strategy to Analyze Rheostat-like Protein Kinase-Dependent Regulation CHEMISTRY & BIOLOGY 2013 FEB 21; 20(2):262-271 Full Text | PMID/PMCID
Foley EA, Kapoor TM Microtubule attachment and spindle assembly checkpoint signalling at the kinetochore NATURE REVIEWS MOLECULAR CELL BIOLOGY 2013 JAN; 14(1):25-37 Full Text | PMID/PMCID
Subramanian R, Kapoor TM Building Complexity: Insights into Self-Organized Assembly of Microtubule-Based Architectures DEVELOPMENTAL CELL 2012 NOV 13; 23(5):874-885 Full Text | PMID/PMCID
Kawashima SA, Takemoto A, Nurse P, Kapoor TM Analyzing Fission Yeast Multidrug Resistance Mechanisms to Develop a Genetically Tractable Model System for Chemical Biology CHEMISTRY & BIOLOGY 2012 JUL 27; 19(7):893-901 Full Text | PMID/PMCID
Firestone AJ, Weinger JS, Maldonado M, Barlan K, Langston LD, O'Donnell M, Gelfand VI, Kapoor TM, Chen JK Small-molecule inhibitors of the AAA plus ATPase motor cytoplasmic dynein NATURE 2012 APR 5; 484(7392):125-129 Full Text | PMID/PMCID
Shimamoto Y, Kapoor TM Microneedle-based analysis of the micromechanics of the metaphase spindle assembled in Xenopus laevis egg extracts NATURE PROTOCOLS 2012 APR; 7(5):959-969 Full Text | PMID/PMCID
Wacker SA, Houghtaling BR, Elemento O, Kapoor TM Using transcriptome sequencing to identify mechanisms of drug action and resistance NATURE CHEMICAL BIOLOGY 2012 MAR; 8(3):235-237 Full Text | PMID/PMCID
Li X, Foley EA, Molloy KR, Li YY, Chait BT, Kapoor TM Quantitative Chemical Proteomics Approach To Identify Post-translational Modification-Mediated Protein-Protein Interactions JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2012 FEB 1; 134(4):1982-1985 Full Text | PMID/PMCID
Tan L, Kapoor TM Examining the dynamics of chromosomal passenger complex (CPC)-dependent phosphorylation during cell division PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2011 OCT 4; 108(40):16675-16680 Full Text | PMID/PMCID
Foley EA, Maldonado M, Kapoor TM Formation of stable attachments between kinetochores and microtubules depends on the B56-PP2A phosphatase NATURE CELL BIOLOGY 2011 OCT; 13(10):1265-U216 Full Text | PMID/PMCID
Li X, Kapoor TM An Optical Switch for a Motor Protein CHEMBIOCHEM 2011 OCT 17; 12(15):2265-2266 Full Text |PMID/PMCID
Wacker SA, Kashyap S, Li X, Kapoor TM Examining the Mechanism of Action of a Kinesin Inhibitor Using Stable Isotope Labeled Inhibitors for Cross-Linking (SILIC) JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2011 AUG 17; 133(32):12386-12389 Full Text | PMID/PMCID
Maldonado M, Kapoor TM Moving Right Along: How PP1 Helps Clear the Checkpoint DEVELOPMENTAL CELL 2011 JUN 14; 20(6):733-734 Full Text | PMID/PMCID
Shimamoto Y, Maeda YT, Ishiwata S, Libchaber AJ, Kapoor TM Insights into the Micromechanical Properties of the Metaphase Spindle CELL 2011 JUN 24; 145(7):1062-1074 Full Text | PMID/PMCID
Maldonado M, Kapoor TM Constitutive Mad1 targeting to kinetochores uncouples checkpoint signalling from chromosome biorientation NATURE CELL BIOLOGY 2011 APR; 13(4):475-U291 Full Text | PMID/PMCID
Weinger JS, Qiu MH, Yang G, Kapoor TM A Nonmotor Microtubule Binding Site in Kinesin-5 Is Required for Filament Crosslinking and Sliding CURRENT BIOLOGY 2011 JAN 25; 21(2):154-160 Full Text | PMID/PMCID
Shimamoto Y, Kapoor TM MECHANOREGULATION Cellular seat belts NATURE 2010 NOV 25; 468(7323):518-519Full Text | PMID/PMCID
Subramanian R, Wilson-Kubalek EM, Arthur CP, Bick MJ, Campbell EA, Darst SA, Milligan RA, Kapoor TM Insights into Antiparallel Microtubule Crosslinking by PRC1, a Conserved Nonmotor Microtubule Binding Protein CELL 2010 AUG 6; 142(3):433-443 Full Text | PMID/PMCID
Tseng BS, Tan L, Kapoor TM, Funabiki H Dual Detection of Chromosomes and Microtubules by the Chromosomal Passenger Complex Drives Spindle Assembly DEVELOPMENTAL CELL 2010 JUN 15; 18(6):903-912 Full Text |PMID/PMCID
Wacker SA, Kapoor TM Targeting a kinetochore-associated motor protein to kill cancer cells PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2010 MAR 30; 107(13):5699-5700 Full Text | PMID/PMCID
Li X, Kapoor TM Approach to Profile Proteins That Recognize Post-Translationally Modified Histone "Tails" JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2010 MAR 3; 132(8):2504-+ Full Text | PMID/PMCID
Islam K, Chin HF, Olivares AO, Saunders LP, De La Cruz EM, Kapoor TM A Myosin V Inhibitor Based on Privileged Chemical Scaffolds ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2010; 49(45):8484-8488 Full Text | PMID/PMCID
2004-2009
Houghtaling BR, Yang G, Matov A, Danuser G, Kapoor TM Op18 reveals the contribution of nonkinetochore microtubules to the dynamic organization of the vertebrate meiotic spindle PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2009 SEP 8; 106(36):15338-15343 Full Text | PMID/PMCID
Foley EA, Kapoor TM Chromosome congression: on the bi-orient express NATURE CELL BIOLOGY 2009 JUL; 11(7):787-789 Full Text | PMID/PMCID
Subramanian R, Kapoor T Meiotic Spindle Self-Organization: One Plus One Equals Only One CURRENT BIOLOGY 2009 APR 14; 19(7):R290-R292 Full Text | PMID/PMCID
Itabashi T, Takagi J, Shimamoto Y, Onoe H, Kuwana K, Shimoyama I, Gaetz J, Kapoor TM, Ishiwata S Probing the mechanical architecture of the vertebrate meiotic spindle NATURE METHODS 2009 FEB; 6(2):167-172 Full Text |PMID/PMCID
Kapitein LC, Kwok BH, Weinger JS, Schmidt CF, Kapoor TM, Peterman EJG Microtubule cross-linking triggers the directional motility of kinesin-5 JOURNAL OF CELL BIOLOGY 2008 AUG 11; 182(3):421-428 Full Text | PMID/PMCID
Fuller BG, Lampson MA, Foley EA, Rosasco-Nitcher S, Le KV, Tobelmann P, Brautigan DL, Stukenberg PT, Kapoor TM Midzone activation of aurora B in anaphase produces an intracellular phosphorylation gradient NATURE 2008 JUN 19; 453(7198):1132-U14 Full Text | PMID/PMCID
Yang G, Houghtaling BR, Gaetz J, Liu JZ, Danuser G, Kapoor TM Architectural dynamics of the meiotic spindle revealed by single-fluorophore imaging NATURE CELL BIOLOGY 2007 NOV; 9(11):1233-U45 Full Text
Loncarek J, Kisurina-Evgenieva O, Vinogradova T, Hergert P, La Terra S, Kapoor TM, Khodjakov A The centromere geometry essential for keeping mitosis error free is controlled by spindle forces NATURE 2007 NOV 29; 450(7170):745-U14 Full Text
Brennan IM, Peters U, Kapoor TM, Straight AF Polo-Like Kinase Controls Vertebrate Spindle Elongation and Cytokinesis PLOS ONE 2007 MAY 2; 2(5):? Article e409 Full Text
Kwok BH, Kapoor TM Microtubule flux: drivers wanted CURRENT OPINION IN CELL BIOLOGY 2007 FEB; 19(1):36-42 Full Text
Peters U, Cherian J, Kim JH, Kwok BH, Kapoor TM Probing cell-division phenotype space and Polo-like kinase function using small molecules NATURE CHEMICAL BIOLOGY 2006 NOV; 2(11):618-626 Full Text
Lampson MA, Kapoor TM Targeting protein stability with a small molecule CELL 2006 SEP 8; 126(5):827-829 Full Text
Kwok BH, Kapitein LC, Kim JH, Peterman EJG, Schmidt CF, Kapoor TM Allosteric inhibition of kinesin-5 modulates its processive directional motility NATURE CHEMICAL BIOLOGY 2006 SEP; 2(9):480-485 Full Text
Gaetz J, Gueroui Z, Libchaber A, Kapoor TM Examining how the spatial organization of chromatin signals influences metaphase spindle assembly NATURE CELL BIOLOGY 2006 SEP; 8(9):924-U41 Full Text
Kapoor TM, Lampson MA, Hergert P, Cameron L, Cimini D, Salmon ED, McEwen BF, Khodjakov A Chromosomes can congress to the metaphase plate before biorientation SCIENCE 2006 JAN 20; 311(5759):388-391 Full Text
Lampson MA, Kapoor TM Unraveling cell division mechanisms with small-molecule inhibitors NATURE CHEMICAL BIOLOGY 2006 JAN; 2(1):19-27 Full Text
Khodjakov A, Kapoor T Microtubule flux: What is it good for? CURRENT BIOLOGY 2005 DEC 6; 15(23):R966-R968 Full Text
Mitchison TJ, Maddox P, Gaetz J, Groen A, Shirasu M, Desai A, Salmon ED, Kapoor TM Roles of polymerization dynamics, opposed motors, and a tensile element in governing the length of Xenopus extract meiotic spindles MOLECULAR BIOLOGY OF THE CELL 2005 JUN; 16(6):3064-3076 Full Text
Kapitein LC, Peterman EJG, Kwok BH, Kim JH, Kapoor TM, Schmidt CF The bipolar mitotic kinesin Eg5 moves on both microtubules that it crosslinks NATURE 2005 MAY 5; 435(7038):114-118 Full Text
Cochran JC, Gatial JE, Kapoor TM, Gilbert SP Monastrol inhibition of the mitotic kinesin Eg5 JOURNAL OF BIOLOGICAL CHEMISTRY 2005 APR 1; 280(13):12658-12667 Full Text
Marcus AI, Peters U, Thomas SL, Garrett S, Zelnak A, Kapoor TM, Giannakakou P Mitotic kinesin inhibitors induce mitotic arrest and cell death in taxol-resistant and -sensitive cancer cells JOURNAL OF BIOLOGICAL CHEMISTRY 2005 MAR 25; 280(12):11569-11577 Full Text
Lampson MA, Kapoor TM The human mitotic checkpoint protein BubR1 regulates chromosome-spindle attachments NATURE CELL BIOLOGY 2005 JAN; 7(1):93-+ Full Text
Mitchison TJ, Maddox P, Groen A, Cameron L, Perlman Z, Ohi R, Desai A, Salmon ED, Kapoor TM Bipolarization and poleward flux correlate during Xenopus extract spindle assembly MOLECULAR BIOLOGY OF THE CELL 2004 DEC; 15(12):5603-5615 Full Text
Solecki DJ, Model L, Gaetz J, Kapoor TM, Hatten ME Par6 alpha signaling controls glial-guided neuronal migration NATURE NEUROSCIENCE 2004 NOV; 7(11):1195-1203 Full Text
Kwok BH, Yang JG, Kapoor TM The rate of bipolar spindle assembly depends on the microtubule-gliding velocity of the mitotic kinesin Eg5 CURRENT BIOLOGY 2004 OCT 5; 14(19):1783-1788 Full Text
Cochran JC, Sontag CA, Maliga Z, Kapoor TM, Correia JJ, Gilbert SP Mechanistic analysis of the mitotic kinesin Eg5 JOURNAL OF BIOLOGICAL CHEMISTRY 2004 SEP 10; 279(37):38861-38870 Full Text
Gaetz J, Kapoor TM Dynein/dynactin regulate metaphase spindle length by targeting depolymerizing activities to spindle poles JOURNAL OF CELL BIOLOGY 2004 AUG 16; 166(4):465-471 Full Text
Lampson MA, Renduchitala K, Khodjakov A, Kapoor TM Correcting improper chromosome-spindle attachments during cell division NATURE CELL BIOLOGY 2004 MAR; 6(3):232-237 Full Text
Peters U, Kapoor TM New probes for microtubule dynamics CHEMISTRY & BIOLOGY 2004 JAN; 11(1):14-16 Full Text
2001-2003
Garrett S, Kapoor TM Microtubule assembly: Catastrophe factors to the rescue CURRENT BIOLOGY 2003 OCT 14; 13(20):R810-R812 Full Text
Canman JC, Cameron LA, Maddox PS, Straight A, Tirnauer JS, Mitchison TJ, Fang GW, Kapoor TM, Salmon ED Determining the position of the cell division plane NATURE 2003 AUG 28; 424(6952):1074-1078 Full Text
Khodjakov A, Copenagle L, Gordon MB, Compton DA, Kapoor TM Minus-end capture of preformed kinetochore fibers contributes to spindle morphogenesis JOURNAL OF CELL BIOLOGY 2003 MAR 3; 160(5):671-683 Full Text
Hotha S, Yarrow JC, Yang JG, Garrett S, Renduchintala KV, Mayer TU, Kapoor TM HR22C16: A potent small-molecule probe for the dynamics of cell division ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2003; 42(21):2379-2382 Full Text
Garrett S, Auer K, Compton DA, Kapoor TM hTPX2 is required for normal spindle morphology and centrosome integrity during vertebrate cell division CURRENT BIOLOGY 2002 DEC 10; 12(23):2055-2059 Full Text
Maliga Z, Kapoor TM, Mitchison TJ Evidence that monastrol is an allosteric inhibitor of the mitotic kinesin Eg5 CHEMISTRY & BIOLOGY 2002 SEP; 9(9):989-996 Full Text
Kapoor TM, Compton DA Searching for the middle ground: mechanisms of chromosome alignment during mitosis JOURNAL OF CELL BIOLOGY 2002 MAY 13; 157(4):551-556 Full Text
Kapoor TM, Mitchison TJ Eg5 is static in bipolar spindles relative to tubulin: evidence for a static spindle matrix JOURNAL OF CELL BIOLOGY 2001 SEP 17; 154(6):1125-1133 Full Text
